Journal: The Journal of Biological Chemistry

Article Title: Base Excision Repair of Tandem Modifications in a Methylated CpG Dinucleotide *

doi: 10.1074/jbc.M114.557769

Figure Lengend Snippet: Single turnover rate constant for DNA glycosylase base excision

Article Snippet: Observed steady-state rate for APE1 incision Expression and Purification of Human Thymine DNA Glycosylase The cDNA encoding human TDG was amplified from pCMV6-XL5-TDG (Origene Technologies) with primers TDG-BamHI-F, 5′-GATCGGATCCATGGAAGCGGAGAACGCGGGC-3′, and TDG-EcoRI-R, 5′-GATCGAATTCTCAAGCATGGCTTTCTTCTTCCTG-3′.

Techniques: Sequencing

Journal: Journal of Central South University Medical Sciences

Article Title: Levels of base excision repair proteins in CD4 + T cells in patients with systemic lupus erythematosus

doi: 10.11817/j.issn.1672-7347.2022.210485

Figure Lengend Snippet: A, C: Comparison of the AID protein expression levels in CD4 + T cells between SLE patients and healthy controls; A, D: Comparison of the TDG protein expression levels in CD4 + T cells between SLE patients and healthy controls; B, E: Comparison of the MBD4 protein expression levels in CD4 + T cells between SLE patients and healthy controls. SLE: Systemic lupus erythematosus.

Article Snippet: In the Human Protein Atlas database, the TDG protein nuclear expression in melanoma maintains a medium-to-high expression and high level is related to adverse outcomes.

Techniques: Comparison, Expressing

Journal: Oncogene

Article Title: Thymine DNA glycosylase as a novel target for melanoma

doi: 10.1038/s41388-018-0640-2

Figure Lengend Snippet: TDG inhibitors decrease clonogenic capacity and cell viability in melanoma cells. a , b Molecular beacon DNA repair assay showing dose-dependent inhibition of TDG glycosylase activity by juglone and closantel, respectively; fluorescence is in arbitrary units (AU); time is in 0.5-s intervals. c , d Conventional TDG glycosylase assays, in which G:T mismatch-bearing double-stranded oligonucleotide substrates and 32 P-labeled at the 3′ end on the mismatched T-containing strand were treated with purified recombinant TDG protein at 37 °C; the reactions were then treated with 100 mM NaOH at 90 °C for 30’ in order to cleave the sugar-phosphate backbone at the AP site and the resulting reaction product was separated from the longer substrate by PAGE. Juglone and closantel were tested at concentrations of 100 nM, 10 μM, and 1 mM. A negative control is included. e , f Cell viability of SK28 melanoma cells treated with increasing doses of juglone and closantel. g , h Clonogenic capacity of SK28 melanoma cells treated with different doses of juglone and closantel or DMSO as a control; i , j corresponding stained plates. All the experiments were performed in duplicate or triplicate and data, normalized to DMSO, are presented as average ± standard deviation. k , l Detection by immunofluorescence of 5caC in juglone- and closantel-treated SK28 cells; DMSO was used as a negative control; nuclei were counter-stained with DAPI. m , n Fluorescence-activated cell sorting (FACS) of propidium iodide-stained Mel501 and SK28 cells, showing that juglone induces cell cycle arrest in G2–M phase and increased S phase/>4 n cells, respectively

Article Snippet: In the Human Protein Atlas database, nuclear expression of TDG protein is also maintained at high-to-medium levels in melanomas; and high expression is associated with unfavorable prognosis (Suppl.

Techniques: Inhibition, Activity Assay, Fluorescence, Labeling, Purification, Recombinant, Negative Control, Control, Staining, Standard Deviation, Immunofluorescence, FACS